ketendoКлиническая и экспериментальная тиреоидологияClinical and experimental thyroidology1995-54722310-3787Endocrinology Research Centre10.14341/ket20117249-56ketendo-4362Research ArticleСтатьиArticlesКомбинированный анализ выявления мутации гена BRAF и экспрессии галектина-3 в дооперационной диагностике рака щитовидной железыCombined analysis of BRAF-gene mutation and galectin-3 expression in preoperative diagnostics of thyroid cancerSemenovD Yu

профессор, заведующий кафедрой общей хирургии ГОУ ВПО СПбГМУ им. акад.И.П.Павлова

-SaraiskyM I-KoloskovaL E-BoriskovaM E-SaburovaI. Yu.-PankovaP A-FechenkoN S-FilippovaO Yu-FarafonovaU V-PesterovaO P-BykovM A-20111506201172ТОМ 7, №2 (2011)4956Copyright © Semenov D.Y., Saraisky M.I., Koloskova L.E., Boriskova M.E., Saburova I.Y., Pankova P.A., Fechenko N.S., Filippova O.Y., Farafonova U.V., Pesterova O.P., Bykov M.A., 20112011Semenov D.Y., Saraisky M.I., Koloskova L.E., Boriskova M.E., Saburova I.Y., Pankova P.A., Fechenko N.S., Filippova O.Y., Farafonova U.V., Pesterova O.P., Bykov M.A.Semenov D.Y., Saraisky M.I., Koloskova L.E., Boriskova M.E., Saburova I.Y., Pankova P.A., Fechenko N.S., Filippova O.Y., Farafonova U.V., Pesterova O.P., Bykov M.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.cet-endojournals.ru/jour/article/view/4362

В ходе работы была изучена возможность использования определения экспрессии галектина-3 методом проточной флуороцитометрии и наличия мутации гена BRAF методом полимеразной цепной реакции (ПЦР) в пунктате из узла щитовидной железы, полученном при тонкоигольной аспирационной биопсии, в диагностике высокодифференцированного рака щитовидной железы на дооперационном этапе. В исследование включен 41 пациент с узловыми образованиями щитовидной железы. По данным цитологического исследования материала, полученного при тонкоигольной аспирационной биопсии, у 20 пациентов выявлен папиллярный рак щитовидной железы, у 17 – фолликулярная опухоль, у 4 – узловой коллоидный зоб. Методом проточной флуороцитометрии была исследована экспрессия галектина-3 и мутация гена BRAF методом ПЦР в материале пункционной биопсии. Чувствительность комбинированного анализа экспрессии галектина-3 и выявления мутации гена BRAF в диагностике рака щитовидной железы составила 95%, специфичность и диагностическая точность также по 95%. Комбинированный анализ экспрессии галектина-3 методом проточной флоуцитометрии и определение BRAF-мутации методом ПЦР могут быть использованы в дооперационной дифференциальной диагностике высокодифференцированного рака и доброкачественных узловых неоплазий щитовидной железы

To assess the utility of combined detection BRAF gene mutation and Gal-3 expression from FNAB specimens in preoperative diagnostic of thyroid gland cancer. 41 patients with thyroid gland nodules were included in the study. By cytological conclusions of evaluated FNA specimens 20 patients had papillar thyroid cancer (PTC), 17 – follicular neoplasia, and 4 – nodular colloid goiter. Gal-3 expression were evaluated by flow cytometry method; BRAF gene mutation were evaluated by PCR. In our study the sensitivity of combined detection BRAF gene mutation and Gal-3 expression was 95%, specificity – 95%, diagnostic accuracy – 95%. Combined detection of BRAF gene mutation by PCR and Gal-3 expression by flow cytometry could be used in preoperative diagnostic of thyroid glad cancer.

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The authors declare that there are no conflicts of interest present.