P-glycoprotein expression in experimental thyroid dysfunction
https://doi.org/10.14341/ket2015311-16
Abstract
Aim.
To study an expression of P-glycoprotein in the liver, lean gut, kidneys, cerebral cortex at experimental dysfunction of the thyroid gland.
Materials and methods.
P-glycoprotein expression was investigated on Chinchilla rabbits by immunohistochemistry method. Serum blood levels of thyroid hormones (general T4, general T3, TTH) was investigated by radioimmune method.
Results.
It was revealed that in rabbits with experimental hyperthyroidism caused by 14-days subcutaneous admi-nistration of thyroxine expression of P-glycoprotein in the liver, lean gut and cerebral cortex was increased. It was shown that in rabbits with experimental hypothyroidism caused by resection of the thyroid gland or administration of thiamazol within 21 days expression of P-glycoprotein in the liver, lean gut and cerebral cortex was decreased.
Conclusion.
Correlation dependence between serum levels of general T4, general T3, TTH and an expression of a P-glycoprotein in the liver, lean gut and cerebral cortex was revealed.
About the Authors
Aleksey Vladimirovich Shchul'kin
Ryazan State Medical University named after academician I.P. Pavlov
Russian Federation
MD, PhD
Russian Federation
MD, PhD
Elena Nikolaevna Yakusheva
Ryazan State Medical University named after academician I.P. Pavlov
Russian Federation
MD, PhD, Professor
Russian Federation
MD, PhD, Professor
Ivan Vladimirovich Chernykh
Ryazan State Medical University named after academician I.P. Pavlov
Russian Federation
MD
Russian Federation
MD
Igor Yur'evich Vinogradov
Ryazan State Medical University named after academician I.P. Pavlov
Russian Federation
MD, PhD
Russian Federation
MD, PhD
Natal'ya Mikhaylovna Popova
Ryazan State Medical University named after academician I.P. Pavlov
Russian Federation
MD, PhD
Russian Federation
MD, PhD
References
1. Якушева Е.Н., Черных И.В., Щулькин А.В., Попова Н.М. Гликопротеин-Р: структура, физиологическая роль и молекулярные механизмы модуляции функциональной активности. // Успехи физиологических наук. – 2014. – Т. 45. – №4 – С. 89–98. [Yakusheva EN, Chernykh IV, Shchul’kin AV, Popova NM. Glikoprotein-P: struktura, fiziologicheskaya rol’ i molekulyarnye mekhanizmy modulyatsii funktsional’noi aktivnosti. Uspekhi fiziologicheskikh nauk. 2014;45(4):89-98. (In Russ).]
2. Кукес В.Г., Грачев С.В., Сычев Д.А., Раменская Г.В. Метаболизм лекарственных средств. Научные основы персона-ли-зационной медицины: руководство для врачей. – М.: ГЭОТАР-Медиа; 2008. [Kukes VG, Grachev SV, Sychev DA, Ramen-skaya GV. Metabolizm lekarstvennykh sredstv. Nauchnye osnovy personalizatsionnoy meditsiny: rukovodstvo dlya vrachey. Moscow: GEOTAR-Media; 2008. (In Russ).]
3. Якушева Е.Н., Щулькин А.В., Бирюкова А.С. Дозозависимое влияние тироксина на функциональную активность глико-протеина-Р в эксперименте. // Биомедицина. – 2012. – №2 – С. 53–60. [Yakusheva EN, Shchul’kin AV, Biryukova AS. Dozozavisimoe vliyanie tiroksina na funktsional’nuyu aktivnost’ glikoproteina-P v eksperimente. Biomeditsina. 2012;2:53-60. (In Russ).]
4. Якушева Е.Н., Бирюкова А.С., Щулькин А.В. Влияние тиамазола на функциональную активность глико-протеина-Р. // Научные ведомости Белгородского государст-венного университета. – 2012. – Т. 10. – №129 – С. 133–138. [Yakusheva EN, Biryukova AS, Shchul’kin AV. Vliyanie tiamazola na funktsional’nuyu aktivnost’ glikoproteina-P. Nauchnye vedomosti Belgorodskogo gosudarstvennogo universiteta. 2012; 10(129):133-138. (In Russ).]
5. Разина А.В., Фролова А.И., Сергеев М.А. Оптимизация метода общей анестезии на кроликах. // Актуальные вопросы ветеринарии и биологии. – 2010. – №1 – С. 32–35. [Razina AV, Frolova AI, Sergeev MA. Optimizatsiya metoda obshchey anestezii na krolikakh. Aktual’nye voprosy veterinarii i biologii. 2010;1:32-35. (In Russ).]
6. Chatterjee S, Malhotra R, Varghese F, et al. Quantitative immunohistochemical analysis reveals association between sodium iodide symporter and estrogen receptor expression in breast cancer. PLoS ONE. 2013;8(1):1-9. doi:10.1371/journal.pone.0054055.
7. Scotto KW, Egan D. Transcriptional regulation of MDR genes. Cytotechnology. 1998;27:257-269. doi: 10.1023/A:1008032716628.
8. Mitin T, Von Moltke LL, Court MH, Greenblatt DJ. Levothyroxine up-regulates P-glycoprotein independent of the pregnane X receptor. Drug Metab Dispos. 2004;32(8):779-782. doi: 10.1124/dmd.32.8.779.
9. Wartenberg M, Ling FC, Müschen M, et al. Regulation of the multi-drug resistance transporter P-glycoprotein in multicellular tumor spheroids by hypoxia-inducible factor (HIF-1) and reactive oxygen species. FASEB J. 2003;17(3):503-505.
10. doi: 10.1096/fj.02-0358fje.
11. Luidens MK, Mousa SA, Davis FB, et al. Thyroid hormone and angiogenesis. Vasc Pharmacol. 2010;52:142-145. doi: 10.1016/j.vph.2009.10.007.
12. Viguerie N, Langin D. Effect of thyroid hormone on gene expression. Curr Opin Clin Nutr Metab Care. 2003;6(4):337-381. doi: 10.1097/01.mco.0000078998.96795.e7.
13. Nishio N, Katsura T, Ashida K, et al. Modulation of p-glycoprotein expression in hyperthyroid rat tissues. Drug Metab Dispos. 2005;33(11):1584-1587. doi: 10.1124/dmd.105.004770.
Supplementary files
Review
For citations:
Shchul'kin A.V., Yakusheva E.N., Chernykh I.V., Vinogradov I.Yu., Popova N.M. P-glycoprotein expression in experimental thyroid dysfunction. Clinical and experimental thyroidology. 2015;11(3):11-16. (In Russ.) https://doi.org/10.14341/ket2015311-16
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